Flow cytometry-based assessment of the detection sensitivity of SOX11 in primary MCL cells. Flow cytometry-mediated analysis of SOX11 (a) showed that MCL cells were specifically stained compared to infiltrating T cells (MCLCD3+) and SOX11-negative malignant cells (FLCD3-). The specificity of the staining was assessed by comparing (b) the relative Δ MFI and (c) the fraction of SOX11 positive cells for each patient sample. Both nodal and leukemic cases of MCL were separated from FL using the SOX11 staining. (d) The sensitivity of the SOX11 staining was estimated by diluting (1:1, 1:10. 1:100 and 1:1000) L-MCL cells in PBMC.
Nordström et al. BMC Cancer 2012 12:269 doi:10.1186/1471-2407-12-269