Figure 3 .
Preparative Real-Imaging cDNA-AFLP Screening.A) cDNA tag patterns were amplified with individual primer combinations Taq+2 and Mse+2 (Taq+2 was labeled with IRD700). After electrophoresis, the gel was transferred and dried on green grid paper and scanned using Odyssey Imaging System (Li-COR Biosciences GmbH). The lanes are occupied by cachexia-associated samples (+) and non-cachectic probes (−) allowing direct visual comparison. Grid coordinates are indicated in millimeters (mm). B) Differentially expressed tag patterns (marked in red boxes) were isolated for sequencing analysis.
Skorokhod et al. BMC Cancer 2012 12:265 doi:10.1186/1471-2407-12-265