Figure 3.

Results of Sanger bisulphite sequencing of patient and control samples. For each gene, DNA of the patient with the maximal methylation within the group of twelve (top) and one with an average degree of methylation (middle) were analysed. Also the result of one randomly selected control (CD19⁺) is shown (bottom). Every row of circles represents the CpG sites of an individual clone. The density of the CpG sites in the genomic sequence is represented by the distances between the circles. Open and filled circles stand for unmethylated and methylated CpG sites, respectively. Grey circles denote point mutations found in SFRP5 (CLL6, CLL9, CD19⁺). Horizontal lines above the circles labelled with “PYRO” indicate the regions quantified by bisulphite pyrosequencing. Arrows indicate the positions of known or presumed transcriptional start sites.