Email updates

Keep up to date with the latest news and content from BMC Cancer and BioMed Central.

Open Access Research article

Expression of proto-oncogene KIT is up-regulated in subset of human meningiomas

Masum Saini1, Ajaya Nand Jha2, Andleeb Abrari2 and Sher Ali1*

Author Affiliations

1 Molecular Genetics Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India

2 Max Super Specialty Hospital, 1, Press Enclave Road, Saket, New Delhi, 110017, India

For all author emails, please log on.

BMC Cancer 2012, 12:212  doi:10.1186/1471-2407-12-212

Published: 6 June 2012

Additional files

Additional file 1:

Figure S1. Detection of KIT transcripts by RT-PCR. (A) RT-PCR results of representative meningioma tumor tissues using primers specific to cytoplasmic domain of the KIT. (B) Confirmation of the quality of cDNA synthesis through RT-PCR using ACTB primers (also served as well loading control). Note the absence of amplicons in the no template control (NTC). NN denotes non-neoplastic. (TIFF 183 kb)

Format: TIFF Size: 183KB Download file

Open Data

Additional file 2:

Table S2. Details of the primers used.

Format: DOC Size: 52KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Figure S2. Agarose gel pictures verifying identity of the BAC clone RP11-586A2. PCR amplification for establishing the identity of BAC clone using primers specific to: (A) cytoplasmic; (B) transmembrane and (C) extracellular domains of the KIT. Note the absence of amplification in the no template control (NTC). The characterized clones were used to determine alterations of KIT in the neoplastic tissue by FISH. C denotes colony and NN, non-neoplastic.

Format: TIFF Size: 183KB Download file

Open Data

Additional file 4:

Table S2. Copy number status of KIT, its signal intensity based on FISH and mutation analysis.

Format: DOC Size: 47KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data