Table 1

Primer sequences
Gene Symbol Gene Name Primer Sequences
Housekeeping genes
ACTB actin, beta F: 5'-AACTGGAACGGTGAAGGTGAC-3'
R: 5'-GACTTCCTGTAACAACGCATCTC-3'
HPRT1 hypoxanthine phosphoribosyltransferase 1 F: 5'-ACATCTGGAGTCCTATTGACATCG-3'
R: 5'-TTAAACAACAATCCGCCCAAAGG-3'
GAPDH glyceraldehyde-3-phosphate dehydrogenase F: 5'-GTGAAGGTCGGAGTCAACG-3'
R: 5'-GGTGAAGACGCCAGTGGACTC-3'
TBP TATA box binding protein F: 5'-GGTGTTGTGAGAAGATGGATGTTG-3'
R: 5'-CCAGATAGCAGCACGGTATGAG-3'
Target genes
CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) F: 5'-ACTAGGCGGTTGAATGAGAGGTTC-3'
R: 5'-CAGGTCTGAGTGTCCAGGAAAGG-3'
EDN1 endothelin 1 F: 5'-CCAACCATCTTCACTGGCTTCC-3'
R: 5'-GTCAGACACAAACACTCCCTTAGG-3'
EEF1E1 eukaryotic translation elongation factor 1 epsilon 1 F: 5'-TGCGGGAGGTTCTTGTTCTG-3'
R: 5'-CTGTTAGACTTGGACCATTGTTTG-3'
GPR56 G protein-coupled receptor 56 F: 5'-CTACAGCCGAAGAATGTGACTC-3'
R: 5'-GCAGAAGCAGGATGTTTGGG-3'
MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae) F: 5'-GATGATGAAGTCCGTGAGGCTATG-3'
R: 5'-TGTTGGTTGCTGCTGAGATGC-3'
NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 F: 5'-CCGTTGGGAATGGTGAGGTC-3'
R: 5'-TTGAGAATGAAGGTGGATGATTGC-3'
OBFC2B oligonucleotide/oligosaccharide-binding fold containing 2B F: 5'-GACGATGTTGGCAATCTG-3'
R: 5'-TGGCTCACTGAAGTTAGG-3'
PML promyelocytic leukemia F: 5'-CCAAGGCAGTCTCACCAC-3'
R: 5'-TTCGGCATCTGAGTCTTCC-3'
SOD2 superoxide dismutase 2, mitochondrial F: 5'-GGTGTCCAAGGCTCAGGTTG-3'
R: 5'-GTGCTCCCACACATCAATCCC-3'

Iofrida et al.

Iofrida et al. BMC Cancer 2012 12:207   doi:10.1186/1471-2407-12-207

Open Data