CD44brightcells show stem-like properties. The CD44bright and CD44dim cells were sorted by FACS from the OVCAR-3 and ES-2 cells before additional Western blotting, sphere and colony formation assays. (A) The CD44bright cells expressed higher levels of Sox2 and Oct3/4 than the CD44dim cells in both cell lines. (B) Larger spheres from the CD44bright cells and cell clusters not qualified as spheres from the CD44dim cells in both cell lines are shown (left panel) with a corresponding histogram (right panel). (C) Colony formation assay also shows more colonies in the CD44bright cells compared to the CD44dim cells in both cell lines (left panel). Three independent colony formation assays show statistically significantly more colonies in the CD44bright cells, in comparison to the CD44dim cells in both cell lines (P < 0.0001, right panel).
Liang et al. BMC Cancer 2012 12:201 doi:10.1186/1471-2407-12-201