Hypoxia pretreatment induced cell proliferation and invasion potential. All the cells were cultivated under either hypoxia or normoxia for 48 hrs before brought back to normoxia condition for proliferation or invasion assays. (A) The cells with hypoxia pretreatment for 48 hrs show significantly faster in growth than the cells always cultivated under normoxia (P < 0.05). (B) Cell invasion assays show that there are significantly more infiltrated cells in the hypoxia pretreated groups than those in the normoxia control groups in both cell lines (P < 0.0001).
Liang et al. BMC Cancer 2012 12:201 doi:10.1186/1471-2407-12-201