Immunohistochemistry (IHC) stain for VEGF showed a lower level of VEGF expression in the xenograft tumor from the animal received MSeA-treatment. A. Control. Target VEGF protein (brown cytoplasm) was extensively and highly expressed in a MDA-MB-231 cell-induced tumor of a representative nude mouse receiving no MSeA administration as control. B. MSeA treatment. VEGF protein expression was at a much lower percentage and level in xenograft tumor of a representative nude mouse receiving oral administration of MSeA at 3 mg/kg/day for 18 days. Scale bar = 50 μm. C. Statistical summary of VEGF positive cells for the control tumor (Mean = 234 cells/field, n = 3) and the MSeA treatment (Mean = 45 cells/field, n = 3) (P < 0.05).
Wu et al. BMC Cancer 2012 12:192 doi:10.1186/1471-2407-12-192