Quantitative detection of CTCs targeting human Alu-sequences. 1 to 100,000 human cancer cells were spiked into 200 μl of tumour-free murine blood. DNA was isolated and qRT-PCR was performed targeting human Alu-sequences. As little as 1 human cell could be detected before negative controls. Measurements were done in triplicates (n.d. = not defined).
Gorges et al. BMC Cancer 2012 12:178 doi:10.1186/1471-2407-12-178