a. Breast cancer cell detection by the AdnaTest - ex vivo. Tumour cells were spiked into 1 ml of fresh mouse blood. Signals for human mRNA of EpCAM, MUC-1, Her2 and Actin were detected at different thresholds for each cell line. No false positive results were seen in control blood samples without human breast cancer cells. Negative controls without mRNA input were performed to exclude false positive results (“RT-“). Positive control was included in the AdnaTest Kit (“PCR+”). b. FACS analysis for EpCAM expression. Breast cancer cells were analysed by fluorescence activated cell sorting for EpCAM expression. Unstained cells are shown in the upper panels. Cells of the MDA-MB-231 line show very weak signals for EpCAM expression compared to MDA-MB-468 and especially cells of the KPL-4 line. c. Western Blot analysis for EpCAM and MUC-1 expression . Cells of the MDA-MB-231 line show weak signals for EpCAM expression compared to MDA-MB-468 and KPL-4 cells, whereas comparable MUC-1 expression levels are seen. Tubulin detection was performed as loading control.
Gorges et al. BMC Cancer 2012 12:178 doi:10.1186/1471-2407-12-178