Open Access Highly Accessed Research article

Novel synergistic antitumor effects of rapamycin with bortezomib on hepatocellular carcinoma cells and orthotopic tumor model

Cun Wang12, Dongmei Gao1, Kun Guo1, Xiaonan Kang2, Kai Jiang2, Chun Sun12, Yan Li1, Lu Sun2, Hong Shu1, Guangzhi Jin12, Haiyan Sun12, Weizhong Wu1 and Yinkun Liu12*

Author Affiliations

1 Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China

2 Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China

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BMC Cancer 2012, 12:166  doi:10.1186/1471-2407-12-166

Published: 4 May 2012

Additional files

Additional file 1:

Table S1. RT-PCR primer sequences and reaction conditions.

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Additional file 2:

Figure S1. Combined treatment with rapamycin and bortezomib inhibits SMMC7721 proliferation. (A) Proliferation of SMMC7721 cells was evaluated by using Cell Counting Kit-8 (CCK-8) at indicated time points. (B) SMMC7721 cells were treated with rapamycin (10 ng/ml), bortezomib (100 nM) or both agents. Cell cycle analysis was carried out after 24 h. One representative experiment of three is shown.

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Additional file 3:

Figure S2. Rapamycin plus bortezomib causes enhanced apoptosis. (A) SMMC7721 cells were treated with rapamycin (10 ng/ml), bortezomib (100 nM) or the combination for 24 or 48 h and stained with Hoechst 33342 (magnification, ×400). (B, C) The quantification of apoptotic cells induced by rapamycin and bortezomib was further confirmed by flow cytometry analysis. *P < 0.01, versus control group; **P < 0.001, versus control group; #P < 0.05, versus bortezomib treatment group at 48 h.

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Additional file 4:

Figure S3. Bortezomib significantly suppressed rapamycin mediated Akt phosphorylation in SMMC7721 cells. (A, B) SMMC7721 cells were cultured in control media, rapamycin (10 ng/ml), bortezomib (100 nmol/L), or rapamycin and bortezomib for 24 h. *P < 0.05, versus control group; **P < 0.01, versus control group; #P < 0.01, versus rapamycin treatment group.

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Additional file 5:

Table S2. Lung metastasis in HCCLM3-R xenograft model.

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