Cas III-ia inhibits cell proliferation and induces morphologic changes in tumor cells. A) Cell viability was determined by the MTT assay. Data represent the mean ± SD (*P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001) of three independent experiments. B) Electron micrograph untreated glioma C6 cells and magnified photographs of 24 h Cas III-ia-treated (5(1μm), 10 (500nm), 15(200nm) and 20 (1μm) μg/ml) glioma C6 cells. N indicates the nucleous, open white arrows point to endoplasmatic reticulum, filled thick arrow to autophagic vacuole, asterisk for margination and condensation of chromatin and black circles show numerous vacuoles observed by electronic microscope.
Trejo-Solís et al. BMC Cancer 2012 12:156 doi:10.1186/1471-2407-12-156