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Copper compound induces autophagy and apoptosis of glioma cells by reactive oxygen species and jnk activation

Cristina Trejo-Solís1*, Dolores Jimenez-Farfan2, Sara Rodriguez-Enriquez3, Francisca Fernandez-Valverde1, Arturo Cruz-Salgado4, Lena Ruiz-Azuara4 and Julio Sotelo1

Author Affiliations

1 Departamento de Neuroinmunología y Neuropatología, Instituto Nacional de Neurología y Neurocirugía, SSA, Insurgentes Sur 3877, México, DF, 14269, Mexico

2 Facultad de Odontología, Universidad Nacional Autónoma de México, México, DF, Mexico

3 Departamento de Bioquímica, Instituto Nacional de Cardiología, SSA, México, DF, Mexico

4 Departamento de Química y Medicina Nuclear, Universidad Nacional Autónoma de México, México, DF, Mexico

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BMC Cancer 2012, 12:156  doi:10.1186/1471-2407-12-156

Published: 27 April 2012

Additional files

Additional file 1: Figure S1:

3-MA inhibits cell death induced by TMZ. The effects of 3-MA on TMZ induced cytotoxicity in C6 glioma cells were determined by the MTT assay in control cells and cells treated with TMZ and TMZ + 5 mM 3-MA for 24 h; data represent the mean ± SD (*P ≤ 0.05, **P ≤ 0.01 and ***P≤ 0.001) of three independent experiments.

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Additional file 2: Figure S2:

Effect of ZVAD on caspase 3 activity. Caspase 3 activity was determined by Western blot in control cells and cells treated with Cas III-ia and Cas III-ia + 50μΜ ZVAD for 24 h. The figures shown are representative of at least three different experiments for each experimental condition.

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Additional file 3: Figure S3:

Persistent activation of JNK upon exposure to Cas III-ia. Total cell lysate from control cells and cells treated with 10 μg/ml Cas III-ia for 6, 12 and 24 h were inmmunoblotted to detect phospho-JNK and total JNK. The figures shown are representative of at least three different experiments for each experimental condition.

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