Figure 4.

TM increases ROS mediated cytotoxicity of various anticancer drugs in ovarian cancer cells. (a) SKOV-3 cells were treated with TM (0, 30 μM) for 24 h, after which the cells were treated for another 24 h with MMC or for another 48 h with 4-HPR or 5-FU in the concentrations indicated. The cell viability was evaluated as described in Methods. Data are expressed as the mean of the triplicate determinations (X ± SD) compared to untreated cells [100%]. (b) SKOV-3 cells were treated with TM (0, 30 μM) for 24 h, after which the cells were treated for another 24 h with doxorubicin (0, 5 μM), MMC (0, 5 μM), 4-HPR (0, 10 μM) or 5-FU (0, 1000 μM). Immunoblotting was carried out with primary antibodies against cleaved PARP, pro-survival marker XIAP or GAPDH as internal control for equal loading. (c) SKOV-3 cells were treated with TM (0, 30 μM) for 24 h, after which the cells were treated for another 24 h with MMC (0, 5 μM) or for another 48 h with 4-HPR (0, 5 μM) or 5-FU (0, 1000 μM) in the presence or absence of radical scavenger ascorbic acid (750 μM).