Table 1

Mutational status of PIK3CA, PTEN, NRAS and BRAF genes in New Zealand Melanoma (NZM) cell lines used for the study

Cell lines

PTEN status

PIK3CA status

NRAS status

BRAF status


NZM6

Exon3 deletion

V600E


NZM34

Exon 5 frameshift

mutation

V600E


NZM43

Exon 1 frameshift

mutation

V600K


NZM30

No identified

mutation in exon

2-9*

V600E


NZM40

H1047R

Q61H


NZM46

H1047R

Q61H


NZM52

H1047R

V600E


NZM10

Q61H


NZM15

Q61H


NZM42

Q61H


NZM3

V600E


NZM12

V600E


*In NZM30, PTEN exon 1 sequencing failed after multiple attempts. The absence of PTEN protein in NZM30 (Figure 2) could be due to a large deletion in exon 1 or epigenetic changes

Kim et al. BMC Cancer 2012 12:141   doi:10.1186/1471-2407-12-141

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