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Comparison of growth factor signalling pathway utilisation in cultured normal melanocytes and melanoma cell lines

Ji Eun Kim16*, Clare Stones12, Wayne R Joseph1, Euphemia Leung1, Graeme J Finlay12, Andrew N Shelling3, Wayne A Phillips5, Peter R Shepherd24 and Bruce C Baguley14

Author Affiliations

1 Auckland Cancer Society Research Centre, The University of Auckland, Auckland, New Zealand

2 Department of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand

3 Department of Obstetrics and Gynaecology, The University of Auckland, Auckland, New Zealand

4 Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Auckland, New Zealand

5 Department of Surgery, Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre and University of Melbourne, St. Vincent's Hospital, Melbourne, VIC, Australia

6 Auckland Cancer Society Research Centre, Private Bag 92019, The University of Auckland, Auckland, New Zealand

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BMC Cancer 2012, 12:141  doi:10.1186/1471-2407-12-141

Published: 4 April 2012

Additional files

Additional file 1:

Table S1 PCR primer and sequencing primer sequences used for the study. The Reference sequences (NCBI) for PTEN-NT_030059.12, PIK3CA-NT_000003.11, NRAS-NC_000001.10 and BRAF-NC_000007.13. # The BRAF exon 11 PCR primers were taken from Davies et al. [9]. *The primers for exon 9-13 of PIK3CA were designed to not match a pseudogene on chromosome 22 [53].

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