Table 2

Primers for quantitative real-time RT-PCR

Gene

Primer

Sequence 5' → 3'

Localization

Annealing temperature


HPRT

HPRT309

5'-TTGCTGACCTGCTGGATTAC-3'

sense

391-410

58°C


HPRT507

5'-CTTGCGACCTTGACCATCTT-3'

antisense

633-652


OPN-a

OPN-a fw 323

5'-ATCTCCTAGCCCCACAGAAT-3'

sense

323-342

58°C


OPN-a rev 508

5'-CATCAGACTGGTGAGAATCATC-3'

antisense

529-508


OPN-b

OPN-b fw 323

5'-ATCTCCTAGCCCCACAGAC-3'

sense

323-341

62°C


OPN-b rev 509

5'-AAAATCAGTGACCAGTTCATCAG-3'

antisense

531-509


OPN-c

OPN-c fw 246

5'-TGAGGAAAAGCAGAATGCTG-3'

sense

246-265

58°C


OPN-c rev 377

5'-GTCAATGGAGTCCTGGCTGT-3'

antisense

396-377


Sequences and localization of primers used in this study correspond to mRNA sequences of HPRT [Genbank: NM_000194.2], OPN-a [Genbank: NM_001040058.1], OPN-b [Genbank: NM_000582.2] and OPN-c [Genbank: NM_001040060.1]

Hahnel et al. BMC Cancer 2012 12:131   doi:10.1186/1471-2407-12-131

Open Data