Influence of JS-K and JS-Kneg on the androgen receptor concentration in human prostate cancer cells. 22Rv1 and LNCaP cells were incubated for 30 hours with or without 5 nM dihydrotestosterone in the presence of JS-K or JS-Kneg. Subsequently cells were lysed and separated by SDS-electrophoresis (15 μg protein per lane). Proteins were transferred onto a nitrocellulose membrane. AR and β-actin (loading control) were visualized by immunodetection.
Laschak et al. BMC Cancer 2012 12:130 doi:10.1186/1471-2407-12-130