Release of NO from JS-K. (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. . (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-Kneg, [C] 2 μM JS-K, [D] 200 μM DETA/NO.
Laschak et al. BMC Cancer 2012 12:130 doi:10.1186/1471-2407-12-130