Open Access Research article

Is there a role for the quantification of RRM1 and ERCC1 expression in pancreatic ductal adenocarcinoma?

Matias E Valsecchi1*, Thomas Holdbrook2, Benjamin E Leiby3, Edward Pequignot3, Susan J Littman1, Charles J Yeo4, Jonathan R Brody5 and Agnieszka K Witkiewicz2

Author Affiliations

1 Department of Medical Oncology, Thomas Jefferson University, 834 Chestnut Street Suite 320, Philadelphia, PA 19107, USA

2 Department of Pathology, Thomas Jefferson University and the Jefferson Pancreas, Biliary and Related Cancer Center, Philadelphia, PA, USA

3 Division of Biostatistics, Department of Pharmacology and Experimental Therapeutics, Thomas Jefferson University, Philadelphia, PA, USA

4 Department of Surgery, Thomas Jefferson University, Philadelphia, PA, USA

5 Departments of Surgery and Pathology, Thomas Jefferson University, Philadelphia, PA, USA

For all author emails, please log on.

BMC Cancer 2012, 12:104  doi:10.1186/1471-2407-12-104

Published: 22 March 2012

Abstract

Background

RRM1 and ERCC1 overexpression has been extensively investigated as potential predictive markers of tumor sensitivity to conventional chemotherapy agents, most thoroughly in lung cancer. However, data in pancreatic cancer are scarce.

Methods

We investigated the mRNA and protein expression of ERCC1 and RRM1 by RT-PCR and immunohistochemistry (IHC) in formalin-fixed, paraffin-embedded pancreatic ductal carcinoma (PDA) tissues. The primary outcome investigated was the association between RRM1 and ERCC1 expression and overall survival (OS) or disease-free survival (DFS).

Results

A total of 94 patients with resected PDA were included in this study. Most of them (87%) received gemcitabine based chemotherapy. Data for OS analysis was available in all cases but only 68% had enough information to estimate DFS. IHC analysis revealed information for 99% (93/94) and 100% of the cases for RRM1 and ERCC1 expression respectively. However, PCR data interpretation was possible in only 49 (52%) and 79 (84%) cases respectively. There was no significant association between high or low expression of either RRM1 or ERCC1, detected by IHC and OS (14.4 vs. 19.9 months; P = 0.5 and 17.1 vs. 19.9; P = 0.83 respectively) or PCR and OS (48.0 vs. 24.1 months; P = 0.21 and 22.0 vs. 16.0 months; P = 0.39 respectively). Similar results were obtained for DFS.

Conclusions

RRM1 and ERCC1 expression does not seem to have a clear predictive or prognostic value in pancreatic cancer. Our data raise some questions regarding the real clinical and practical significance of analyzing these molecules as predictors of outcomes.