Nucleus Detection. Smoothing and adaptive histogram equalization are performed to alleviate variability in H&E stained image and to obtain better contrast. "RG - B" conversion followed by thresholding characterizes the areas where nuclei exist. Morphological closing operation is performed to fill holes and gaps within nuclei, and a watershed algorithm segments each individual nuclei. The segmented nuclei are constrained by their shape, size, and average intensity and epithelial cell classification (green pixels) provided by the overlaid IR image.
Kwak et al. BMC Cancer 2011 11:62 doi:10.1186/1471-2407-11-62