Table 4

Mutational Spectrum of Oncogenes and Tumor Suppressor Genes

Gene

GBC (%)

EHCC (%)

IHCC (%)

Method

Refs.


CTNNB1/

β-CATENIN

5

SEQ

[33]

9

0

SEQ

[34]

0

SEQ

[35]


KRAS

38

PCR-SSCP

[5]

20

PCR-RFLP

[16]

19

PCR-RFLP

[32]

3

15

SEQ

[3]

10

PCR-SSCP

[15]

54

SEQ

[6]

45

SEQ

[2]

48

SEQ

[36]

0

0

13

OncoMap


BRAF

33

SEQ

[7]

22

SEQ

[2]

0

0

0

SEQ & GLCR

[8]


EGFR

9

18

20

SEQ

[9]

6

SEQ

[10]

12

5

10

IHC and FISH

[37]


PIK3CA

4

0

9

SEQ

[11]

13

0

0

OncoMap


ERBB2/

HER-2

16

5

0

IHC and FISH

[37]


P16INK4A

31

SSCP

[16]

62

55

numerous

[13]

88

numerous

[6]


TP53

36

SSCP

[16]

33

PCR-SSCP

[15]

37

SEQ

[14]


SMAD4

16

PCR-SSCP

[12]

55

13

IHC

[38]


STK11/LKB1

6

SEQ

[39]


Abbreviations: GBC; gallbladder carcinoma, IHCC; intra-hepatic cholangiocarcinoma, EHCC; extra-hepatic cholangiocarcinoma, SEQ; sequencing, PCR-SSCP; polymerase chain reaction single-strand confirmation polymorphism, RFLP; restriction fragment length polymorphism, GLCR; gap ligase chain reaction.

Deshpande et al. BMC Cancer 2011 11:60   doi:10.1186/1471-2407-11-60

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