Figure 8.

Interferon response of prostate cancer cells. (a) IFN-β production from mock-infected and RSV-infected RWPE-1 and LNCaP cells was measured at 12 h and 24 h post-infection. Amount of IFN-β deduced from the ELISA assay was expressed as pg/ml and each value represents mean ± standard deviation for three determinations. (b) RSV infectivity in untreated (UT) and IFN pre-treated cells was measured by plaque assay at 24 h post-infection. The values are fold reductions in the RSV titer following IFN pre-treatment compared to untreated cells. Mean ± standard deviation for three determinations are shown. (c) Plaque assay showing RSV infectivity in untreated (UT) and IFN pre-treated RWPE-1 and LNCaP cells. Culture supernatant collected from RSV infected cells were added to CV-1 cells at various dilutions (1 × 104 - 1 × 106 dilutions). (d) Plaque assay showing RSV infectivity in untreated (UT) and IFN pre-treated RWPE-1 and PC-3 cells at 24 h post-infection. Culture supernatant collected from RSV infected cells was added to CV-1 cells at various dilutions (1 × 105 and 1 × 108 dilutions). For figures (c) and (d) the plaques were observed on methyl-cellulose after crystal-violet staining. Please note that figures (c) and (d) are two separate experiments.

Echchgadda et al. BMC Cancer 2011 11:43   doi:10.1186/1471-2407-11-43
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