Sustained tumor remission after RSV administration and subdued immune response to RSV challenge. (a) RSV was administered (via I. T - four injections at 2-day intervals) to xenograft tumor (generated from luciferase expressing LNCaP-Luc2 cells). Following 4 injections, the mouse was observed everyday for a period of 44 days for tumor recurrence. Real-time bioluminescence imaging of xenograft tumors in the live animal was performed with Xenogen IVIS system. (b) Levels of interferon-γ (IFNγ) & interleukin-10 (IL-10) in spleen homogenate of normal, C57BL/6J mice (non-tumor bearing) infected with RSV (4 injections of RSV, 2 days apart -106 pfu/mouse via i.p.) were measured (by ELISA) at the day-14 following last injection. Cytokines were also measured in xenograft prostate tumor (subcutaneous tumor generated using LNCaP cells) 48 hr after RSV injections via I.T. The representative results are from duplicate experiments with similar values.
Echchgadda et al. BMC Cancer 2011 11:43 doi:10.1186/1471-2407-11-43