Table 2

Growth curves in glioma cells exposed to vehicle or ketone bodies

21% O2

3OHB

AcAc

LiCl

3OHB + AcAc

3OHB + LiCl


U87MG

94.9 ± 9.9

109.7 ± 10.3

104.5 ± 11.7

107.3 ± 10.1

100.8 ± 10.0

U251MG

100.2 ± 5.8

97.4 ± 5.5

97.0 ± 6.9

96.1 ± 5.4

90.2 ± 5.0

LNT-229

98.4 ± 4.7

100.6 ± 3.7

109.5 ± 7.9

104.0 ± 3.6

95.7 ± 4.0

T98G

96.3 ± 4.8

87.6 ± 4.0

91.2 ± 7.8

86.1 ± 5.3

88.0 ± 4.1

A172

97.1 ± 6.0

100.3 ± 5.7

99.9 ± 7.4

84.9 ± 10.7

90.7 ± 5.6

LNT-229 scrambled sh

99.9 ± 9.5

109.2 ± 6.0

103.7 ± 5.3

102.2 ± 6.2

97.5 ± 6.8

LNT-229 p53sh

98.3 ± 5.3

102.3 ± 3.2

96.5 ± 2.4

95.1 ± 2.4

95.0 ± 2.8


0.1% O2

3OHB

AcAc

LiCl

3OHB + AcAc

3OHB + LiCl


U87MG

97.7 ± 4.9

115.8 ± 5.8

115.2 ± 4.9

108.8 ± 4.2

104.0 ± 5.7

U251MG

96.6 ± 2.7

102.6 ± 5.0

98.1 ± 2.5

92.1 ± 5.4

86.9 ± 3.0

LNT-229

93.6 ± 3.5

103.2 ± 4.9

104.4 ± 3.3

97.0 ± 3.3

91.0 ± 5.1

T98G

98.8 ± 10.1

100.2 ± 9.1

97.3 ± 8.7

101.7 ± 7.3

91.5 ± 9.9

A172

107.9 ± 15.6

93.2 ± 9.2

95.8 ± 7.4

101.0 ± 8.7

91.6 ± 6.8

LNT-229 scrambled sh

99.6 ± 5.4

104.5 ± 4.7

105.4 ± 6.0

98.9 ± 4.6

103.9 ± 8.2

LNT-229 p53sh

94.8 ± 2.5

107.4 ± 4.2

107.6 ± 5.4

102.9 ± 4.0

96.0 ± 4.5


Cells were exposed to 3-hydroxybutyrate (3OHB, 5 mM), acetoacetate (AcAc, 5 mM), lithium chloride (LiCl, 5 mM) or both for 36 h at 21% or 0.1% O2 and subsequently stained with crystal violet. Data are expressed as percentages (mean and standard deviation) relative to untreated control cells.

Maurer et al. BMC Cancer 2011 11:315   doi:10.1186/1471-2407-11-315

Open Data