Optimal PMA treatment concentration for pMEK and pERK activation. Cells were plated and grown overnight in media containing 1% fetal calf serum, except for control cells that were grown in regular media containing 10% fetal calf serum. Cells were then treated with increasing amounts of PMA (50-200 nM) for two hours. Additional control cells were incubated for one hour with the same concentration of DMSO as present in the PMA samples (DMSO). Cells were lyzed, and equal amounts of total protein of each sample were subjected to immunoblotting with antibodies against ERK and pERK. Two representative experiments are shown.
Taherian et al. BMC Cancer 2011 11:293 doi:10.1186/1471-2407-11-293