Table 2

Oligonucleotide primer sequences used for the amplification of different regions of HPV and internal control.

Primers

Primer Sequences

Location

Annealing

temperature

(ºC)

Amplimer size

(bp)

Reference

MY09

5'-GCM CAG GGW CAT AAY AAT GG-3'

L1

55

450

[49]

MY11

5'-CGT CCM ARR GGA WAC TGA TC-3' where M = A + C, W = A + T, Y = C + T, R = A + G).

GP5+

5'-TTT GTT ACT GTG GTA GAT ACT AC-3'

L1

40

145

[51]

GP6+

5'-CTT ATA CTA AAT GTC AAA TAA AAA-3'

HPV16 E6 (F)

5'-GAA ACC GGT TAG TAT AAA AGC AGA C-3'

53-78

55

503

[49]

HPV16 E6 (R)

5'-AGC TGG GTT TCT CTA CGT GTT CT-3'

557-533

HPV16 E7 (F)

5'-CAA TAA TAT AAG GGG TCG GTG GA-3'

480-500

55

468

HPV16 E7 (R)

5'-TTT TTC CAC TAC AGC CTC TAC AT-3'

945-923

[49]

β-globin (F)

5'-GAA GAG CCA AGG ACA GGT AC-3'

4-23

55

268

[49]

β-globin (R)

5'-CAA CTT CAT CCA CGT TAC ACC-3'

273-256

p53 Exon 5 (F)

5'-TAC TCC CCT GCC CTC AAC AA-3'

316-355

55

184

[48]

p53 Exon 5 (R)

5'-CAT CGC TAT CTG AGC AGC GC-3'

499-480

Hedau et al. BMC Cancer 2011 11:27   doi:10.1186/1471-2407-11-27