Additional file 4.

COBRA results of three SIP1 putative promoter regions in paired HCC samples. Figure S1. BstUI restriction analysis of the P1 region amplified by SIPM1dF/SIPM1iyR1 and SIPM1iF/SIPM1iyR1 semi-nested primer pairs. Fourteen out of 29 HCC samples (48%) are methylated. Figure S2. BstUI restriction analysis of the P2 region amplified by SIPM2iyF1/SIPM2iR and SIPM2iyF1/SIPM2iyR2 semi-nested primer pairs. One out of 26 HCC samples (4%) is methylated. Figure S3. TaqI restriction analysis of the P3 region amplified by SIPM3dF/SIPM3dR and SIPM3iF/SIPM3iR nested primer pairs. Ten out of 23 HCC samples (43%) are methylated.

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Acun et al. BMC Cancer 2011 11:223   doi:10.1186/1471-2407-11-223