Enhanced apoptotic cell death of PC3 cells treated with reovirus and docetaxel in combination. PC3 cells were treated with reovirus MOI 1 (open bars), 20 nM docetaxel (wide diagonal stripes) or both agents together (narrow diagonal stripes) for 24 h (a) or 48 h (b) before staining with Annexin V (A) and propidium iodide (PI) and analysing using flow cytometry. Untreated cells (dark grey bars were included as a negative control). Addition of each agent alone resulted in an increased late apoptotic/necrotic population (A+/PI+) and concomitant reduction of intact cells (A-/PI-) compared to untreated cells. There was a dramatic increase in this effect at 48 h when the two agents were combined.
Heinemann et al. BMC Cancer 2011 11:221 doi:10.1186/1471-2407-11-221