Live virus is required for efficient cell kill and enhanced cell kill with docetaxel. PC3 cells were treated with live (diamonds), heat-inactivated (squares), or UV-inactivated (triangles) virus for 48 h at the MOI indicated at which point survival was measured by MTS assay (a). PC3 cells were also treated with docetaxel alone (diamonds), or docetaxel with live reovirus (squares), heat-inactivated reovirus (triangles) or UV-inactivated virus (crosses) for 48 h at the concentration and MOI indicated. Survival was measured at this time by MTS assay. The combination of live reovirus with docetaxel enhanced cell kill compared with docetaxel alone (P < 0.0001, two-way ANOVA), however this enhancement of cell kill was not apparent when heat inactivated or UV-inactivated reovirus was used (b). Data for both are normalized to uninfected controls (MOI 0) and are representative of three independent experiments.
Heinemann et al. BMC Cancer 2011 11:221 doi:10.1186/1471-2407-11-221