Effects of JP, Dox and DT on PAPR-1 cleavage. AsPC-1 cells were treated with JP (10 μM), Dox (10 μM) or DT (10 μM), either alone or in combination. PARP-1 cleavage was measured by Western blotting. The total cell lysate was subjected to SDS-PAGE and immunoblotted with PARP-1 antibody, which recognizes full-length (116 kDa) and cleaved (89 kDa) PARP-1 protein. Expression of GAPDH (37 kDa) was analyzed as loading control on a separate gel. Data are representative of two independent experiments with similar results.
Awasthi et al. BMC Cancer 2011 11:15 doi:10.1186/1471-2407-11-15