Additional file 2.
Treatment with curcumin or FLLL32 did not significantly alter pERK1/2 or total ERK1/2 levels. Canine (OSA8) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or increasing concentrations of FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for pERK1/2 (Thr202/Tyr204), total ERK1/2, and β-actin was performed. Experiments were repeated two times.
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Fossey et al. BMC Cancer 2011 11:112 doi:10.1186/1471-2407-11-112