FLLL32 induced activation of caspase 3/7, PARP cleavage, and apoptosis of OSA cell lines. A) Canine (OSA8, OSA16, and D17) and human (SJSA and U2OS) OSA cell lines were treated with media, DMSO, curcumin, or FLLL32 for 24 hours. Apoptosis was assessed by measuring active caspase-3/7 using the SensoLyte® Homogeneous AMC Caspase-3/7 Assay kit. Experiments were performed in triplicate and repeated two times. Statistical analysis of the caspase 3/7 activity was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.*p < 0.05 B) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for PARP and β-actin was performed. Experiments were repeated two times.
Fossey et al. BMC Cancer 2011 11:112 doi:10.1186/1471-2407-11-112