Treatment with curcumin or FLLL32 decreased the proliferation of OSA cell lines. A) Canine (OSA8, OSA16, and D17) or human OSA cell lines (SJSA and U2OS) were treated with vehicle, curcumin, or FLLL32 for 72 hours. Proliferation was analyzed using the CyQUANT® cell proliferation assay kit. Proliferation values are listed as a percentage of DMSO control. Experiments were performed in quadruplicate and repeated two times. Statistical analysis of cell proliferation was performed using the Student's t-test. P values of < 0.05 were considered statistically significant.*p < 0.05 B) Canine and human OSA cell lines were treated with DMSO or FLLL32 for 72 hours and analyzed with CyQUANT® to determine proliferation as a percentage of DMSO control. IC50s for FLLL32 were calculated for each cell line using a log curve. Experiments were performed in quadruplicate and repeated two times.
Fossey et al. BMC Cancer 2011 11:112 doi:10.1186/1471-2407-11-112