Open Access Highly Accessed Research article

The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines

Stacey L Fossey1, Misty D Bear1, Jiayuh Lin23, Chenglong Li4, Eric B Schwartz4, Pui-Kai Li34, James R Fuchs4, Joelle Fenger5, William C Kisseberth5 and Cheryl A London13*

Author Affiliations

1 Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA

2 Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH 43205, USA

3 Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA

4 Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA

5 Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH 43210, USA

For all author emails, please log on.

BMC Cancer 2011, 11:112  doi:10.1186/1471-2407-11-112

Published: 28 March 2011

Additional files

Additional file 1:

Curcumin and FLLL32 downregulated the expression of STAT3 protein without significant inhibition of STAT3 mRNA transcript levels. OSA8 cells were treated with 10 μM curcumin or 10 μM FLLL32 and were collected at 4 and 24 hours after treatment, and real-time PCR for STAT3 mRNA was performed. Bars represent STAT3 relative expression (2^-Delta Ct). Experiments were performed in triplicate and repeated three times. The difference between treatment groups and DMSO control group was analyzed using the Students t test. P values of < 0.05 were considered statistically significant. There was no statistical significance between the treatment groups.

Format: PDF Size: 215KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Treatment with curcumin or FLLL32 did not significantly alter pERK1/2 or total ERK1/2 levels. Canine (OSA8) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or increasing concentrations of FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for pERK1/2 (Thr202/Tyr204), total ERK1/2, and β-actin was performed. Experiments were repeated two times.

Format: PDF Size: 308KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data