Table 4

IHC analysis confirms HAX-1 overexpression in tumors

Patient

HAX-1

ER


Normal

Tumor

Tumor


Cytoplasm

Nucleus

Cytoplasm

Nucleus

Nucleus


1

0

-

2

-

+


2

0/1

-

1

-

-


3

1

-

1

-

-


4

0

-

0

-

-


5

0

-

1

-

-


6

0

-

1

-

-


7

1

-

0

+

+


8

0

-

2

+

+


9

0

-

1

-

-


10

2

-

2

-

+


11

1

-

1/2

-

+


12

0

-

2

+

+


13

0

-

2

-

-


14

0

-

2

+

+


15

0

-

3

-

-


Immunohistochemical analysis of the 15 matched pairs of normal/tumor samples shows in most cases significant overexpression of HAX-1 in tumors. Up-regulation was observed in cytoplasm as well as nuclei of the tumor cells. Staining intensity was estimated as follows: negative = 0; weak = 1; intermediate = 2; and strong = 3. Nuclear staining was categorized as negative or weak (0-1:"-") and intermediate-strong (2-3: "+"). Strong nuclear HAX-1 staining was associated with strong ER immunopositivity. Fisher's exact test indicates that nuclear staining of HAX-1 and ER is significantly related (P = 0.01).

Trebinska et al. BMC Cancer 2010 10:76   doi:10.1186/1471-2407-10-76

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