Schematic representation of the RBSP3 (A), NPRL2/G21/NPR2L/TUSC4 (B), and RASSF1A (C) genes. Exon-intron structure of known mRNAs is shown. Coding and non-coding mRNA regions are depicted by dark and light grey boxes, respectively. Primers and probes designed for qPCR are shown by arrows and boxes above mRNAs. A. RBSP3 (3p21.3, AP20 region). Forward primer crossed the 1st/2nd exons boundary, reverse primer and probe were located in the 2nd exon. B. NPRL2 (3p21.3, LUCA region): Forward primer crossed the 5th/6th exons boundary, reverse primer crossed the 6th/7th exons boundary, probe was located in the 6th exon. C. RASSF1A (3p21.3, LUCA region): Forward primer crossed the 1st/2nd exons boundary that provides specificity to isoforms A and E. Reverse primer crossed the 2nd/3rd exons boundary that provides specificity to isoforms A, B and D. Combination of forward and reverse primers provided specificity to isoform A only. Probe was located in the 2nd exon.
Senchenko et al. BMC Cancer 2010 10:75 doi:10.1186/1471-2407-10-75