CDK4 and MDM2 analysis. (A) Immunohistochemistry with anti-CDK4 antibody showing strong and quite diffuse nuclear staining of fusiform cells (× 20). (B) Immunohistochemistry with anti-MDM2 antibody showing a more focal nuclear positivity. (C) Fluorescence in situ hybridization of one neoplasic cell: high nuclear amplification of CDK4 (red signals) and MDM2 (green signals), with more than 20 copies (× 40).
Sabatier et al. BMC Cancer 2010 10:645 doi:10.1186/1471-2407-10-645