aCD4S presensitization followed by irradiation enhanced Bax expression. Hela cells were plated in T25 flask at 5 × 105/well/5 ml CM overnight, treated with aCD4S for 2 days, followed by 4 Gy γ-irradiation, plated into 6-well plate at 2.5 × 105cells/well with 3 ml CM for 2 additional days. Western blot analysis was performed to evaluate the expression of the pro-apoptotic protein Bax.
Wang et al. BMC Cancer 2010 10:60 doi:10.1186/1471-2407-10-60