Additional file 2.
The level of PDGFR-alpha activation and of PDGF-B expression is similar in wild type and NG2-KO glioma cells. (A) Western blot of two wild-type (WT) and two NG2-KO (KO) PDGF-B-induced glioma cultures for the indicated proteins. The ratio between the level of activated PDGFR-alpha and total PDGFR-alpha is shown in the histogram. Values were normalized to the WT-1 culture. (B) Histogram shows the levels of PDGF-B mRNA relative to the housekeeping gene Rpl41 in wild type and NG2-KO glioma cultures. Values represent the means of real-time PCR quantifications for two independent cultures per condition.
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Terrile et al. BMC Cancer 2010 10:550 doi:10.1186/1471-2407-10-550