Anti-proliferative effects of TStrC. a) CFSE assay to measure the anti-proliferative properties of TStrC. PBMC were stimulated with IL-2 (100 IU/ml) and OKT3 (1 μg/ml). Black bars show the proliferation of PBMC in presence of TStrC, grey bars indicate the proliferation of PBMC in presence of MSC. For each TStrC, a dose dependent inhibition of proliferation was seen (n = 10). b) To mimick the hypoxic tumour-microenvironment, PBMC proliferation assays in presence of TStrC were also conducted under low-oxygen tension (1%). The inhibitory effect of TStrC was maintained under hypoxic conditions (n = 4). c) The anti-proliferative property of TStrC also affected NK cells of different HLA mismatched donors (n = 3). NK cells were isolated using CD56+ magnetic beads and stimulated with 100 IU/ml IL-2.
Johann et al. BMC Cancer 2010 10:501 doi:10.1186/1471-2407-10-501