Table 1

Sources and dilutions of primary antibodies related to the Vitamin D metabolism used in this study for immunohistochemistry

Antibody

Clone

Manufacturer

Time of incubation (min)

Dilution

Antigen retrieval (min)


VDR

9A7γE10.4

Calbiochem, Germany

overnight

1:50

30


CYP27B1

C12

Santa Cruz, USA

60

1:200

30


CYP24A1

C18

Santa Cruz, USA

overnight

1:75

30


Lopes et al. BMC Cancer 2010 10:483   doi:10.1186/1471-2407-10-483

Open Data