Table 3

Quantification cycle (Cq) values of candidate reference genes and target miRNAs (in triplicate) in colorectal tissues

Reference gene

Cq Range

Cq Min

Cq Max

Mean Cq ± s.d.


let-7a

8.02

24.25

32.27

28.31 ± 1.60


miR-16

4.75

21.85

26.60

23.61 ± 1.00


miR-26a

6.96

21.09

28.05

23.61 ± 1.49


miR-345

9.37

25.14

34.51

27.43 ± 1.35


miR-425

10.01

24.65

34.66

27.53 ± 1.36


miR-454

9.64

27.76

37.40

30.14 ± 1.75


RNU48

9.71

22.08

31.79

26.34 ± 2.09


Z30

12.58

26.32

38.90

31.17 ± 2.83


Target miRNA


miR-21

14.21

19.57

33.78

24.82 ± 4.07


miR-31

18.12

20.81

38.93

29.36 ± 3.75


miR-143

12.19

15.95

28.14

23.01 ± 2.84


miR-145

14.03

18.06

32.09

24.84 ± 2.79


Chang et al. BMC Cancer 2010 10:173   doi:10.1186/1471-2407-10-173

Open Data