Additional file 3.
MS spectra and chromatograms of three typical proteins which showed no significant changes in breast cancer. MS/MS spectra of unique peptides (left panels) and their ICAT-labeled parent ion chromatograms (right panels). Heavy and light ICAT-labeled peptides are shown with gray- and black-colored peaks, respectively. (A) Alpha-2-HS-glycoprotein with unique peptide sequence, C§NLLAEK. (B) A vitamin D-binding protein with VC+SQYAAYGEK. (C) A histidine-rich glycoprotein with VSPTDC+SAVEPEAEK. C§ and C+ are cysteines labeled with a heavy 13C-reagent and a light 12C-reagent, respectively.
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Kang et al. BMC Cancer 2010 10:114 doi:10.1186/1471-2407-10-114