Figure 1.

Mini-array, sandwich-type, enzyme-linked immunosorbent assay for chemokine detection. (A) Rows and columns in the image represent wells, of 96-well polystyrene plate, in the bottom of which monoclonal antibodies (mAbs) were spotted in a 3 × 3 pattern. After detection the plate was imaged with cooled charge-coupled device (CCD) camera. (B) Location of anti-chemokine mAb spots in the mini-array and CCD camera image of a 3 × 3 mini-array within a single well. Among nine of the studied chemokines it is CCL19 (also previously known as MIP-3β) which was significantly decreased in sera of patients with preterm labor (see the text for full details).