Table 1

Oligonucleotide primer used in the amplification of GAD1 exons.

Primer

Forward

Reverse


Exon 1

dGCCCCATTTATTTCCCAGCC

dGCACAGCTCTCGCTTCTCTT

Exon 2

dGAAAACCATTGTCCTCCACC

dGCCTGTCGGCTCACAGATT

Exon 3

dACCAGCTTCTTGTGCCATAG

dATCTACTGGCTAGCATGGGG

Exon 4

dATTCCATGTCTGAGCAGCCT

dACTGTTACTGCCCAAGCTTG

Exon 5

dGCCGTTTGCCTTCAAGATAG

dAGAACCACTGGGACTGAACT

Exon E

dACCAGTATCTCCTCGCCATG

dTTGGGAGGCCCCTGGAAATT

Exon 6

dACCCAACTACAAATACTAAACC

dAATAGGAAGTCAGGGTATCC

Exon 7

dGAGACACCAGCTCAGCGTTC

dCTGCAACAAACAGAGGCTCG

Exon 8

dGTCGGGGATGCTTTCTCCATG

dCTCAGTACATTGTGCCAAGC

Exon 9

dCAAGCTGCTAATGGTCTGTT

dGTCTCATATTATCAAGGACTG

Exon 10

dCACAATTCTTCTTCCTGTGA

dTGGGGAGGAGCTTGAGGCAA

Exon 11

dACAATCAGTGTGGGCTGAAC

dGAAGCAAACTTAGACCGAAA

Exon 12

dCTTGAGTTGGAATGGGTGTT

dACTGCAAAGAGACCCCACGT

Exon 13

dTCCTTCCAAGCAGCCTAGTT

dGTGATATATCTTTGCCCCTC

Exon 14

dGACAGCATAGCCTTCCCAAA

dCATGTTGCCAGAAGCTTCAG

Exon 15

dGGTTTGGGAACAGCTTTCTC

dTTCCCCCACTAGAAAGGCAC

Exon 16

dGTTAAAAAGAGAGGGTGTTC

dCCCTCAATGAAATGGCCTGT


Lynex et al. BMC Neurology 2004 4:20   doi:10.1186/1471-2377-4-20

Open Data