Additional file 2.
Figure S2. Examples of real-time PCR amplifications. Three real-time PCR assays were designed to detect the JCV T (A) and VP1 (B) sequences and the contamination of samples with standard DNA (C). The reactions were performed in the absence or presence of standard DNA (2.0 x 108 to 0.8 copies per reaction). Relative fluorescence is plotted against cycle number. These PCR assays were capable of detecting at least 4 copies of JCV DNA per reaction under the same conditions. The data are representative of three independent experiments.
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Nakamichi et al. BMC Neurology 2012 12:121 doi:10.1186/1471-2377-12-121