Intracellular Ca2+ release mediates PC2 translocation to the PM. (A-B) Quantitative analysis of a number of PM PC2-positive rPT cells following treatment with 1 μM ionomycin (A), 1 μM thapsigargin (Tg) (B), or thapsigargin with 10 μM BAPTA (B). (C) Single cell Ca2+ recording of two rPT cells loaded with Fura-2/AM treated with 100 μM ouabain followed by sequential addition of 2 mM Mn2+ to the recoding medium. (D) Statistical analysis of slope decrease in non-oscillating (gray bar) and oscillating cells (black bar) in response to ouabain after Mn2+ addition to the recording medium. Values are the mean ± SEM, *P < 0.05, ***P < 0.001 vs. control, and #P < 0.001 vs. treatment.
Miyakawa et al. BMC Nephrology 2013 14:34 doi:10.1186/1471-2369-14-34