Figure 2.

PC2 trafficking to the PM in rat proximal tubule (rPT) cells. (A-F) Immunocytochemistry of PC2 in rPT cells treated with control (A), 100 μM ouabain (C), or 20 nM bradykinin (E). Scale bars, 20 μm. Localization profile of PC along a line (as indicated) through the center of a single rPT cell treated with control (B), ouabain (D), or bradykinin (F). Gray areas represent PM regions. (G) Quantitative analysis of a number of PM PC2 positive rPT cells following indicated treatments. Values are the mean ± SEM and ***P < 0.001 vs. control.