Change in body volume status in Pax8-rtTA/LC1/floxed ETA (iETA) mice comparing before and after vehicle or doxycycline (DOX) treatment. Animals were fed a normal (0.3%) Na diet for 7 days followed by a high (3.2%) Na diet for 7 days, then treated with DOX, and the diets repeated; measurements were obtained on the 7th day of each diet. Panel A shows change in body weight, Panels B and C show changes in total body water and extracellular fluid volume, respectively, as assessed by impedance plethysmography, and Panel D shows the change in hematocrit. N = 8 each data point. *p < 0.05 vs. –DOX, same diet.
Stuart et al. BMC Nephrology 2012 13:166 doi:10.1186/1471-2369-13-166